CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Maternal fat overfeeding programs alterations in PPARalpha pathways related to lipid metabolism in livers from rat fetuses and offspring
Autor/es:
FORNES, DAIANA; JAWERBAUM, ALICIA; HEINECKE, FLORENCIA; WHITE, VERÓNICA
Lugar:
CABA
Reunión:
Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017
Institución organizadora:
SAIC
Resumen:
We previously found liver lipid overaccumulation, decreased mRNA levels of acyl CoA oxidase (AcO) and carnitine palmitoyl transferase-1 (CPT-1) and no response to leptin- induced lipid catabolic actions in fetuses from rats fed a fatty diet (SFD rats). PPARalpha is a positive regulator of liver lipid catabolism through the upregulation of AcO and CPT-1 and lipid trafficking through perilipin 2 (plin2).Aim: to analyze whether liver from fetuses and offspring from the control and SFD group respond to the lipid catabolic effects of the PPARalpha activator clofibrate (clof). Methods: Female rats were fed with standard (controls) or saturated fat diet (27% fat) since they were 6 week-old (SFD rats). After 8 weeks, they were mated with control males. Control and SFD rats were euthanized at gestational day 21 or allowed to deliver and their offspring euthanized at 140 days of age. Offspring?s and fetal livers were cultured (3h) with or without clof (20 M). Lipid levels (Triglycerides (TG), phospholipids (PL), cholesterol (Ch), free fatty acids (FA) and cholesteryl esters (CE)) were assessed by TLC, and Plin2, ACO and CPT1 mRNA levels by qPCR. Results: Clof decreased liver lipid levels in control fetuses (FA and CE: 25%, p