Diets enriched in olive oil regulates the expression of PPAR target genes in the fetal liver in a rat model of gestational diabetes mellitus (GDM) induced by intrauterine programming

FORNES, DAIANA; ALICIA JAWERBAUM; VERÓNICA WHITE; CAPOBIANCO, EVANGELINA

CABA

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

In a rat model of GDM induced by intrauterine programming, we previously found sex-dependent alterations in PPAR target enzymes involved in lipid accretion or oxidation as well as increased prooxidant/proinflammatory markers in the fetal liver. Aim: To address whether maternal diets enriched in olive oil prevent the alterations in lipid metabolizing enzymes in the fetal liver and whether there are changes in the expression of nuclear factor erythroid-derived-2 like 2 (NRF2), a PPAR target gene relevant in the regulation of antioxidant enzymes. Methods: Pregestational diabetic rats (F0) were obtained by neonatal streptozotocin administration. F0 rats were mated with healthy males. No treatments were performed in the F1 offspring until the 3rd month of age, when the rats were mated with healthy males. During F1 pregnancies the rats were fed with a standard diet supplemented or not with 6% olive oil. Maternal metabolic parameters and the mRNA levels of the genes codifying fatty acid synthase (FAS), acyl CoA oxidase (ACO) and NRF2 in the fetal liver were evaluated (by RT-PCR) on day 21 of pregnancy. Results: Maternal olive oil treatment did not prevent maternal hyperglycemia but reduced maternal triglyceridemia (21%, p