Changes in microRNAs that target PPARs and lipid accretion is sex-dependent in the fetal liver of rats with GDM

DAIANA FORNES; VERÓNICA WHITE; ALICIA JAWERBAUM; CAPOBIANCO EVANGELINA

Nyborg

Congreso; 49th Annual Meeting, Diabetes and Pregnancy Study Group (DPSG), EASD; 2017

GDM is a prevalent disease that impairs fetal metabolism and development and programs metabolic alterations in the offspring. PPARs are nuclear receptors crucial in development and metabolic regulation. We have previously characterized a GDM diabetic rat model induced by developmental programming. In this model, diabetes is spontaneously induced during pregnancy when the offspring of mild diabetic rats are mated with control males. Aiming to address putative alterations in the liver of fetuses of GDM rats, we analyzed lipid content, PPAR levels and microRNAs that target PPARs in a sexdependent manner. GDM was spontaneously induced in the offspring (F1) of rats with mild diabetes (induced by neonatal streptozotocin administration in F0). Male and female fetuses were evaluated on day 21 of pregnancy. Male fetuses showed increased levels of triglycerides and cholesterol in their livers (p