Androgen excess in utero produce long term alterations on ovarian functions

GISELLE ADRIANA ABRUZZESE; ALICIA BEATRIZ MOTTA; MARIA FLORENCIA HEBER

BUENOS AIRES

Congreso; Joint Meeting of Bioscience Societies; 2017

SAIC

An adverse environment during prenatal life programs the developing embryo and may conduct to diseases during the adult life. In previous works we have shown that prenatal hyperandrogenism is able to induce endocrine abnormalities that mimics those observed in human polycystic ovary syndrome at pubertal age. The aim of this work is to study the long term impact of prenatal hyperandrogenism on reproductive functions, at adult life.Pregnant rats were hyperandrogenized with testosterone and a Control group was obtained by vehicle injection. To study the long term effect of fetal programming caused by androgen excess on reproductive functions,prenatally hyperandrogenized (PH) female offspring(N=100) and Controls(N=80) were characterized according to the estrous cycle as ovulatory (PHov) and anovulatory (PHanov) phenotypes at adult age and gonadotropins serum levels (LH and FSH) were measured. To study the effect of androgen excess on ovarian functions we quantified by qPCR the mRNA levels of steroidogenic enzymes (SF1, StAR and P450aromatase) and the oxidant/antioxidant balance as levels ofMDA and GSH, respectively.At adult life Control rats showed (100%) regular estrous cycle, 51% of the PH group showed irregular estrous cycles (PHov), whereas 49% presented anovulatory cycles (PHanov). LH and FSH were not affected in any of the PH groups. Regarding steroidogenesis, StARSF1 and P450 aromatase levels were decreased in the PHanov group as compared to controls (p