Anandamide stimulates the migration of human endometrial fibroblasts stromal cells and tubulogenesis of human first trimester trophoblast.

BELTRAME JS; CAÑUMIL VA; RIBEIRO ML; MERESMAN G; SORDELLI MS

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias; 2017

SAIC

Vascular adaptations at the maternal-fetal interface are crucial for successful implantation and placentation. Decidual cells regulate the activity of the endovascular trophoblast and actively participate in spiral artery remodeling. Anandamide (AEA) is the main endocannabinoid described so far related to the process of implantation. AEA levels are primarily regulated by fatty acid amide hydrolase (FAAH), its degrading enzyme. Dysregulations in AEA levels and FAAH activity have been associated with implantation failure and decidual defects. However, the role of AEA in vascular processes at implantation sites has not been elucidated. Thus, the aims of the present study were: 1) to analyze the correlation between FAAH expression and a known mediator of vascular processes (IL-10); 2) to investigate the effect of AEA on endometrial stromal cell migration and trophoblast tubulogenesis. First, Wistar females were sacrificed in days 5 (Dp5) and 10 (Dp10) of gestation. Uteri (Dp5) and decidua (Dp10) were collected and processed for qRT-PCR and western blot. Then, T-hESC (human endometrial stromal cells) and HTR-8/SVneo (human first trimester cytotrophoblast) cell lines were incubated with AEA 1 nM and tested for migration (8 and 10 h) or tubulogenesis (6 h), respectively. The mRNA expression of IL-10 augmented in deciduas from Dp10 compared to Dp5 uteri (p