PARTICIPATION OF CANNABINOID RECEPTOR 1 (CB1) ON LIPOPOLYSACCHARIDE (LPS)-INDUCED PRETERM LABOR

SCHANDER JULIETA; HERRERO LO GIUDICE FELISA MARÍA ; AISEMBERG JULIETA; BARIANI MARIA VICTORIA; FRANCHI ANA MARIA; CELLA MAXIMILIANO; WOLFSON MANUEL

Buenos Aires

Congreso; Reunión Conjunta de Sociedades de Biociencias 2017, LXII Reunión Científica Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2017

Sociedad Argentina de Investigación Clínica

The endocannabinoid system (eCS) is comprised by a large family of amides, esters and ethers of long chain polyunsaturated fatty acids, most of which are ligands for cannabinoid receptors (CB1 and CB2) and participate in numerous physiological and pathological processes during pregnancy. Anandamide (AEA) is synthesized on demand from membrane lipid precursors by the action of N-acylphosphatidylethanolamine-specific phospholipase D (NAPE-PLD). The biological activity of AEA is terminated by its intracellular degradation by a fatty acid amide hydrolase (FAAH). Prematurity is the leading cause of mortality and morbidity in neonates. It is well known that premature deliveries are associated with infectious process. We have previously developed a murine model of LPS-induced preterm labor to mimic the pathological process implicated in prematurity due to infections. The aim of this study is to explore the role of the eCS on LPS-induced preterm labor. A study of uterine and cervix explants was performed to characterize and also to investigate the eCS role in mediating the effects of LPS on proinflammatory mediators. We have already shown by western blot the presence of eCS (FAAH, NAPE-PLD, CB1, CB2) in mouse pregnant uterus and in this study we observed that eCS is also expressed in cervix explants. Also uterine FAAH expression was diminished by LPS treatment (p