Autoantibodies from breast cancer patients promote migration in MCF-7 human breast adenocarcinoma cells

PELEGRINA LAURA TATIANA; GABRIEL FISZMAN; AZAR MARIA EUGENIA; CRESTA MORGADO CARLOS; SALES MARIA ELENA

Viña del Mar, Chile

Congreso; 9º Latinoamerican Congress of Immunology; 2009

Latinamerican Association of Immunology

The muscarinic acetylcholine receptors (mAChR) are expressed in human breast tumor tissue and in MCF-7 cells, a human mammary adenocarcinoma cell line, while mAChR are absent in normal mammary cells MCF-10A. We have demonstrated the presence of anti-mAChR antibodies (Abs) in breast cancer patients that stimulates MCF-7 cells proliferation. Metastases is the first cause of death in cancer patients, the aim of this work is to investigate the participation of mAChR in tumor cells migration as well as the ability of  anti-mAChR Abs from breast cancer patients in T1N0Mx (tumor diameter<2cm, without lymph node metastasis) on MCF-7 migration.  We demonstrated that the muscarinic agonist carbachol (CARB) stimulated tumor cell migration in a concentration dependent-manner. The action of the maximal effective concentration (10-10M) was 135±12 % (p<0.001 vs.control, cells without treatment). Pre-incubation of cells with 10-9M atropine (AT) prevented CARB effect. IgG from T1N0Mx patients stimulated MCF-7 cells migration in a concentration-dependent manner being  10-8M  the maximal effective concentration and promoting migration by 200±25% (p<0,001 vs.control. Control IgG (from normal patients) did not modify MCF-7 cells migration. We can conclude that anti-mAChR Abs can be modulating tumor progression by stimulating migration that could be inducing tumor metastases.