CONICET | Buscador de Institutos y Recursos Humanos

CEFYBO 02669

CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS

Unidad Ejecutora - UE

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Título:

Experience-dependent retinal protection against acute ischemia

Autor/es:

DEVOUASSOUX, JULIÁN D; DORFMAN, DAMIÁN; ROSENSTEIN, RUTH E; MILNE, GEORGIA A; ARANDA, MARCOS L; CHIANELLI, MÓNICA S; DIEGUEZ, HERNÁN H; GONZÁLEZ FLEITAS, MARÍA F

Reunión:

Congreso; Southamerican Glia Meeting: The role of glial cells in health and disease of the Nervous System; 2017

Resumen:

Ischemia is a key component of several retinal diseases that are leadingcauses of irreversible blindness. At present, there are no effective strategiesto prevent retinal ischemic damage. Enriched environment (EE) refers toconditions that facilitate or enhance sensory, cognitive, motor, and socialstimulation relative to standard (laboratory) conditions. The aim of thepresent work was to analyze whether the exposure to EE prevents acute retinalischemic damage.Adult male Wistar ratswere housed instandard environment (SE) or EE for 3 weeks. SE consisted instandard laboratory cages, housing 2 animals/cage, and EE consisted in bigcages housing 6 animals and containing several food hoppers, wheels anddifferent objects repositioned once/day and fully substituted once/week. In SEand EE, animals were provided with food and water adlibitum. After 3weeks of SE or EE, retinal ischemia was induced by increasing intraocularpressure above120 mm Hg for 40 min.Immediately after ischemia, both groups were further housed in SE for 2weeks.Retinal function (electroretinography, ERG),histology (H&E), Mullercells (glial fibrillary acidic protein, (GFAP immunoreactivity)) and retinalganglion cell (RGC) number(Brn3a immunohistochemistry) were assessed.In animalshoused in SE, ischemia induced a significant decrease in ERG a- and b- waveamplitude and oscillatory potentials, whereas the previous exposure to EEprevented these functional alterations. Two weeks after ischemia, a significantdecrease in the total retinal thickness and the number of Brn3a(+) cells, aswell as an increase in Muller cell GFAP levels werefound in retinas fromanimals housed in SE, whereas in ischemic retinas from animals housed inEE,theseparameters were significantly preserved. These resultssuggest that the exposure to EE prevents retinal alterations induced by acuteischemia.