CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Rejection of murine T lymphoma due to immunization with costimulatory molecules transfected tumor cells.
Autor/es:
DI SCIULLO, MARÍA PAULA; HERSCHLIK, LETICIA; WALDNER, CLAUDIA; MONGINI, CLAUDIA
Lugar:
Viña del Mar. Chile
Reunión:
Congreso; 9th Latin American Congress of Immunology; 2009
Resumen:
The aim was to develop cancer vaccines employing irradiated tumor cells transfected with T-cell costimulatory molecules. BALB/c mice (n=5-10/group) were inoculated i.p. with 1x106 T-cell lymphoma cells (LBC) transfected with CD40, CD40L or with combination of CD40, CD40L and CD80 and irradiated with 3000 cGy, or controls (PBS and irradiated mock transfected tumor cells), twice at 7-day intervals. Mice were challenged 7 days after the last immunization with 1x106 tumor cells i.p. All immunized groups rejected the tumor. Our results indicated survival rates of  70-100% for LBC.CD40, 40-60% for LBC.CD40L and combination groups and 55-70% for LBC. Mice immunized with LBC showed a significant difference in survival regarding the challenged groups (p <0.0374), as the experimental plots LBC.CD40 (p <0.0067) and LBC.CD40L (p <0.0499) (Log Rank test). In addition, there were higher levels of IFN-ã in the supernatant of splenocytes immunized with LBC.CD40 (473pg/ml), LBC.CD40L (310pg/ml) and combined inoculation (266pg/ml) respect to LBC (49pg/ml) and normal (44pg/ml). Also, specific cytotoxicity by Jam’s method was 19% for LBC.CD40, 14% for LBC and 7% for normal mice. Therefore, immunization with irradiated cells transfected with CD40 has a protective effect, prolonging the survival of immunized mice respect to LBC immunized mice and unimmunized.