CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Development of experimental Foot-and-Mouth-Disease Vaccines: effect of two adjuvants and role of macrophages in early protective responses
Autor/es:
QUATTROCCHI, V; LANGELLOTTI, C; PAPPALARDO, S; OLIVERA, V; DI GIACOMO, S; ROOIJEN VAN, N; MONGINI C; WALDNER C; ZAMORANO, P
Lugar:
Viña del Mar, Chile
Reunión:
Congreso; Immunochile 2009 (IX Congreso Latinoamericano de Inmunología); 2009
Institución organizadora:
Asociación Latinoamericana de Inmunología (ALAI)
Resumen:
Foot and Mouth Disease is an acute disease caused by Foot and Mouth Disease Virus (FMDV). In this report, we studied the protection and early immune response induced in mice by adjuvanted vaccines with inactivated FMDV. The presence of IMS802 or ISA206 adjuvants yielded increased protection against viral challenge and low, but significantly higher, FMDV-specific antibody levels (measured by ELISA), as compared to the control group vaccinated with FMDV without adjuvants. In vivo macrophage depletion in mice inoculated with vaccines containing IMS802 or ISA206, decreased the percentages of protected animals after virus challenge, indicating a central role of this cell population in the response elicited by the vaccines. Accordingly, opsonophagocytosis (measured by flow cytometry), was augmented in these mice. On the other hand, IMS802 plus inactive FMDV was evaluated in cattle. All vaccinated animals were protected against viral challenge at 4 and 7 days post vaccination and macrophages levels were increased as compared to the control group vaccinated with inactive FMDV. These results demonstrate the ability of the studied adjuvants to enhance the FMDV-specific protective responses of inactivated vaccines, and the main role of opsonization and in vivo antibody-mediated phagocytosis in the early protective immune responses against FMDV infection.