PPARs and mTOR interact in the rat decidua during early organogenesis

S. ROBERTI, H. SATO, R. HIGA, A. JAWERBAUM

Puerto Varas

Congreso; VII SLIMP Latin American Symposium on Maternal Fetal Interaction and Placenta; 2017

During embryo organogenesis, before the establishment of a mature placenta, the decidua serves for the embryonic histotrophic nutrition. Peroxisome proliferator activated receptors (PPARs) are nuclear receptors essential for development that regulate metabolic processes. Mammalian target of rapamycin (mTOR) signaling is relevant in embryo nutrition and growth. Objectives: Aiming to assess whether PPARs and mTOR signaling pathways are interrelated in rat decidua during early organogenesis, we studied the effect of in vivo inhibition of mTOR, PPARgamma and PPARdelta signaling. Methods: Female Wistar rats received subcutaneous injections of rapamycin (mTOR inhibitor), T0070907 (PPARgamma inhibitor), GSK0660 (PPARdelta inhibitor) or vehicle during days 7, 8, and 9 of pregnancy. On day 9, decidua was explanted and level of proteins phosphorylated by the mTORC1 pathway (ribosomal protein S6 (RPS6) and eukaryotic initiation factor 4E-binding protein 1 (4E-BP1)) and by the mTORC2 pathway (glucocorticoid-inducible kinase 1 (SGK1)), as well as adipophilin (a PPAR target)) were evaluated by western blot. Results: Rapamycin administration increased decidua PPARgamma (36%, P