CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Oxidative stress is involved in the increase in POMC expression by supraphysiological glucose levels in a corticotroph cell line
Autor/es:
CALANNI JS; VECINO, CV; CYMERYNG, CB; REPETTO, EM; SANCHEZ PUCH, S; MERCAU ME; WISZNIEWSKI, M
Lugar:
Mar del Plata
Reunión:
Congreso; LXI REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE INVESTIGACIÓN CLÍNICA; 2016
Institución organizadora:
Sociedad Argentina de Investigación Clínica
Resumen:
Glucocorticoids (GCs) are theultimate effectors of the hypothalamus pituitary adrenal axis (HPA)exerting regulatory effects on carbohydrate and lipid metabolismon multiple targettissues. An increasing number of studies suggest that hyperactivationof the HPA could be involved in the etiology of themetabolic syndrome. In order to examine this issue,we previously studied the effects of a hypercaloric diet (30% sucrosein the drinking water, SRD) on the HPA function in rats, in early stages of the development of IR, when no significant decrease in insulinsensitivity was detected by an insulin tolerance test. Our resultsshowed increased circulating levels of ACTH and corticosterone in these animals. Analysis of pituitarytissues indicated higher levels of POMC and oxidative stress (OxS)parameters in rats of the SRD group. The aim of the following studies was toanalyze the mechanisms involved in the regulation of POMC expression incorticotroph cells. Luciferase activity was determined in a corticotroph cell line (AtT20) transfected with the reporter plasmidsPOMC-LUC or HO1-LUC and incubated with 10 mM glucose. Generation of reactive oxygen species was also measured in thesecells. Our results indicated that luciferase activity, determined incells transfected with POMC-LUC, was significantly increased in thepresence of 10mM glucose (p