Changes in ovarian gene expression induced by 3-methylcholathrene and prevented by alpha-naphthoflavone

LOMNICZI, ALEJANDRO; GALARZA, ROCÍO ALEJANDRA; RHON CALDERÓN, ERIC ALEJANDRO; FALETTI, ALICIA GRACIELA

Mar del Plata (Pcia. de Buenos Aires)

Congreso; LXI Reunión Anual de la Sociedad Argentina de Investigación Clínica; 2016

SAIC-SAI-SAFE-NANOMEDAR-AACYTAL

3-Methylcholanthrene (3MC), a polyaromatic hydrocarbon, is an environmental pollutant that causes reproductive toxicity. Previous studies showed that 3MC alters the ovarian function byaffecting the follicle integrity in rodents. The aim of the present work was to study the effect of daily exposure to 3MC on ovarian gene expression involved in folliculogenesis, cell cycle and xenobiotic metabolism. Ovarian tissue from immature female rats, daily injected with 3MC (0.1 and 1 mg/kg) and/or α-naphthoflavone (αNF, 80 mg/kg) for 20 days, were used. Both doses of 3MC increasedthe mRNA expressionof Akt1, Cdk2, Dnajb6, Igf2, Calr, Hspa8, Icam1, Ddr2, Dll1, Hes1, Notch2, Jag1, Cyp1a1, Cyp1b1, Ddx5, Foxn3 and Sp1 (p