molecular por pcr de Escherichia coli patógenas en terneros de la cuenca lechera de Villa Maria (Córdoba)

PICCO, N; ALUSTIZA, F; BELLINGERI, R; TIRANTI KA; VISSIO CA; LOMBARDELLI JA; MOREIRA AB; LOUGE EB; LUCHETTI CA; LARRIESTRA AA; TERZOLO HB; VIVAS AA

Huerta Grande, Córdoba

Congreso; XVII Jornadas Científicas de Biología de Córdoba; 2009

Sociedad de Biología de Córdoba

In Córdoba, the bovine herd is the first production. Diarrhea caused by E. coli generates economic loss around 20%. Some bovine bacterial strains could infect humans. There is no recent information about neonatal diarrhea in Córdoba. The aim of this study was to obtain current data about pathogenic E. coli strains causing diarrhea in neonatal calves from Villa Maria. Samples of rectal swabs of calf less than 45 days old (n=61), were transported in Stuart and cultured on Mc Conkey agar. Lac + colonies compatible with E coli were biochemically characterized and DNA was extracted by heating. Virulence factors (LT, STa, F17, F5 and eae) were identified by PCR.  Results and Discussion: There was a 36.1% pathogenic strain: 16.42% EHEC, 9.84% ETEC, 1.64% EPEC and only 8.20% were F17+. A 63.9% of non-pathogenic E. coli was found. The predominance of EHEC strains could impact on public health. Furthermore the currency of ETEC and EPEC could affect the productive capacity of artificial bringing up calves.