Cepas patógenas de Escherichia coli en lechones neonatos de un criadero intensivo porcino de Córdoba

ALUSTIZA, F; PICCO, N; BELLINGERI, R; ASHWORTH, G; BERARDO DB; MOREIRA AC; LOUGE EC; LUCHETTI CA; GRECO CB; TERZOLO HC; VIVAS AA

Huerta Grande, Córdoba

Congreso; - XVII Jornadas Científicas de Biología de Córdoba; 2009

Sociedad de Biología de Córdoba

Neonatal porcine diarrhea is a major cause of economic losses in pig production. One of the main etiologic agents is Escherichia coli. Both enterotoxigenic (ETEC) and enteropathogenic (EPEC), associated with immunosuppression were the cause of stress management. The aim of this work was to characterize by PCR the strains of E. coli that produce diarrhea in neonatal piglets of pig intensive breeding in Córdoba. Samples of rectal swabs (n=58) transported in Stuart were analyzed, cultured on Mc Conkey agar. Lac + colonies compatible with E coli were biochemically characterized and DNA was extracted by heating. Virulence factors (LT, STb, STa, F18, eae) were identified by PCR. Result and Discussion: The findings showed a 1.72% of EPEC (eae+) and 24.14% of ETEC, which were 50% STb+, 21.4% LT+, 14.3% STa+; 7.15 % F18+ and 7.15%  F18+ STb+. A 74.14% of non-pathogenic E. coli was found. It shows a predominance of ETEC currency. These results show that, in this intensive breeding, with controlled environment and good prophylaxis (vaccination), the presence of pathogenic strains E. coli remains possibly by stress management.