CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Lysophosphatidic acid increases the production of pivotal mediators of decidualization and vascularization
Autor/es:
BELTRAME JS; SORDELLI MS; SZMELC AT; CELLA M; PEREZ MARTINEZ S; FRANCHI AM; RIBEIRO ML
Lugar:
Mar del Plata
Reunión:
Congreso; VI Latin American Symposium on Maternal Fetal Interaction and Placenta and V Latin American Symposium on Reproductive Immunology Meeting; 2015
Institución organizadora:
SLIMP y LASRI
Resumen:
Although the human population is in continuous growth and will probably reach nine billion people by 2016, global statistics indicate that 15% of couples have infertility problems. This rate is compounded considering that only 50-60% of pregnancies exceeds 20 weeks of gestation, being implantation failure the cause of pregnancy loss in 75 % of cases.The decidual reaction and the formation of new vessels in the uterus are two crucial processes during embryo implantation. Based on published data we postulate that lysophosphatidic acid (LPA) might be a lipid promoter of vascularization and decidualization. Previously, we observed that the pharmacological blockade of LPA3, an LPA receptor, provoked aberrant embryo spacing and increased embryo resorption with negative consequences in the vascularization and decidualization reactions at the sites of embryo implantation. Thus, we investigated LPA3 potential signaling pathways. Both cyclooxygenase (COX) and nitric oxide synthase (NOS) are known enzymes involved in implantation. Uterine slices on day 5 of gestation (day of implantation) were isolated and incubated with LPA, DGPP (a highly selective antagonist of LPA3) and COX and NOS selective and non-selective inhibitors.. We observed that LPA, through the activation of LPA3 receptor, increased inducible NOS activity and the production of COX-2 derived prostaglandin E2. In addition, nitric oxide and prostaglandins presented a crosstalk under the effect of LPA. LPA also induced the expression of markers of vascularization (IL-10) and decidualization (IGFBP-1) through LPA3 receptor and these signaling pathways involved iNOS and COX-2.Taking into account our previous data, the in vitro results suggest that the LPA3 receptor and its ligand, LPA, are involved in the process of vascularization and decidualization that take place during embryo implantation.