Cholinergic activation modulates breast tumor-induced production of cytokines by human dendritic cells.

OROÑO M; LOMBARDI G,; SALES M E

Buenos Aires

Congreso; LXIII Reunión Anual de la Sociedad Argentina de Inmunología; 2015

SAI

Background: We have previously described the presence of non neuronal cholinergic system in human dendritic cells (DC). Taking into account that a deficient functional activity of DC has been reported in tumor bearers we investigated the role of cholinergic receptors (ChR) activation in DC-MCF-7 tumor interaction regarding cytokines production. Methods: DC were obtained from human monocytes and then cultured during 24 h in the absence or presence of lipopolysaccharide to obtain immature or mature DC (iDC or mDC). Then both populations were treated during 24 h with different concentrations of MCF-7 tumor extract (MTE). Afterwards, DC were stimulated with the cholinergic agonist carbachol during 1 h. The production of cytokines in DC culture supernatants was quantified by ELISA. Results: the treatment of iDC with increasing doses of MTE triggered a potent up-regulation of interleukin (IL)-10 production while the effect on IL-12 or tumor necrosis factor alfa (TNF-α) levels was less potent (495±19%**; 295±28%*; 269±20%*, respectively; **p