CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
CYCLOPHOSPHAMIDE INCREASES THE RELEASE OF EXOSOMES FROM A MURINE T-CELL LYMPHOMA
Autor/es:
COCOZZA F; MENAY F; TSACALIAN R; LISAZO J; GRAVISACO M; VENDRELL A; WALDNER C; MONGINI C
Lugar:
Buenos Aires
Reunión:
Congreso; II French-Argentinean Immunology Meeting; 2015
Institución organizadora:
Sociedad Argentina de Inmunología
Resumen:
Cells ubiquitously secrete exosomes at a basal level but are specifically induced in response to various stress stimuli as cytotoxic drugs. Exosomes are 60-100 nm membrane vesicles involved in cell?cell communication, expressing marker proteins such as Alix and Tsg-101, cellular proteins including tumor antigens, and immunologically molecules such as MHC molecules, costimulatory molecules and heat shock proteins (Hsp?s). The aim of the present work was to determine if cyclophosphamide (CTX) modulate exosome release by a murine T-cell lymphoma. We determined that the concentration of 3 mM was optimal to induce stress without loss of viability. Exosomes were isolated by a serial of differential centrifugation and ultracentrifugation at 100.000xg from supernatants produced by equal amount of LBC cells cultured 24h at steady state and CTX stressed conditions. The exosomal yield was measured by total SAI 2015 50 exosomal protein measurement by Bradford and the expression of Tsg-101 by flow cytometry. Under CTX stress, the exosome secretion from LBC cells was increased as measured by both methods. The normalized total protein expression was significantly enhanced (p