Cliptox increased the protection levels induced by an FMDV inactivated

QUATTROCCHI, V; OLIVERA, V; LANGELLOTTI, C; PAPPALARDO, S; MONGINI, C; PORTUONDO, P; ZAMORANO, P

ERICE, SICILIA ITALIA

Congreso; 2008 Open Session of the The European Commission for the control of Foot-and-Mouth disease (EuFMD) Standing Technical Committee; 2008

FAO. The European Commission for the control of Foot-and-Mouth disease (EuFMD)

Introduction: Foot and Mouth Disease (FMD) is an acute disease caused by Foot and Mouth Disease Virus (FMDV) which causes important economy losses, this is why it is necessary to obtain a vaccine that stimulates a rapid and long lasting protective immune response. Cliptox TM is a mineral microparticle that in earlier studies has shown adjuvant activity against different antigens. In this study we have evaluated the capacity of inducing specific and protective immune response using a formulation that includes inactivated foot and mouth diseases virus (iFMDV) and microparticled adjuvant (Cliptox)   Materials and methods: BALB/C mice were vaccinated subcutaneously with experimental vaccines using iFMDV alone or formulated with Cliptox. The antibodies and cytokines were detected by ELISA. Protection against viral challenge was assessed.   Results: It was demonstrated that iFMDV-Cliptox TM is non toxic by using egg embryos. On the other hand it stimulates a specific antibody response detected in mucosal and in sera. Also, cellular profile compatible with a Th1 response was detected. iFMDV-Cliptox TM vaccine increased protection percentages compared to the iFMDV group in the mouse model, which demonstrates that the adjuvant could be useful in protective vaccine formulation against this disease in target animals.   Conclusion: Our results indicate that the incorporation of Cliptox into FMDVi vaccine induces an increase of the specific and protective humoral and cellular immune response in mice.