Cancer Immunotherapy based on the regional lymph nodes stimulation with an attenuated salmonella.

VENDRELL A; GRAVISACO M; MONGINI C; WADNER CI

Buenos Aires

Congreso; Congreso de la Sociedad Argentina de Farmacología Experimental , 2007; 2007

Sociedad Argentina de Farmacología Experimental (SAFE)

When tumors spread beyond the primary site, they often come to rest in the regional lymph nodes. The hostLs immune response, both to cancer and to infectious diseases is also initiated in these nodes. The aim of this study is to develop an immunotherapy protocol to promote the regression of a subcutaneous (s.c) tumor using a recombinant bacteria attenuated salmonella as an anticancer drug. Balb/c mice were s.c inoculated with LM3 tumor cells (mammary adenocarcinoma murine). When tumors were palpable, animals were immunized with bacteria by s.c route, for three times, in either the peritumoral or intratumoral and the periganglionar areas. Mice treated with bacteria display a significant reduction in the tumor size and a significant increase in the medium survival time (p < 0.05, Logrank test). In addition, immunization induced draining lymph node cells to produce significant levels of IFN ƒÁ (p < 0.05), indicating the presence of a Th1-type cytokine response. Our results demonstrated an efficient antitumor therapeutic response elicited by this novel therapy.