CAMP AND ITS METABOLITES AS BOVINE SPERM CAPACITATION INDUCERS

ALONSO, CARLOS AGUSTÍN ISIDRO; OSYCKA SALUT, CLAUDIA ELENA; CASTELLANO, LUCIANA; DAVIO, CARLOS; PÉREZ MARTINEZ, SILVINA

Chascomús

Jornada; XVI ANNUAL MEETING OF THE ARGENTINE BIOLOGY SOCIETY; 2014

Sperm capacitation has been associated with several molecular events such as: increase in intracellular cAMP levels, kinases activation and protein phosphorylation. Our previous results show that cAMP efflux and activation of A1 adenosine (ADO) receptors are critical in the regulation of bicarbonate-induced sperm capacitation. Moreover, cAMP may act as an autocrine/paracrine factor in the extracellular compartment. In the present work we aim to deepen into the action pathway of cAMP and its metabolites (5´AMP; ADO). For that, we assayed in vitro capacitation in the presence of the cAMP or its metabolites and PLC and PKA inhibitors. The sperm capacitation was evaluated by CTC and LPC-induced acrosome reaction assays. Our results showed that 10 nM of cAMP or 5?AMP induce sperm capacitation, but it is necessary 10 uM ADO to accomplish the same effect. Furthermore, when spermatozoa were incubated with non-capacitating concentrations (NCC) of cAMP plus ADO, they undergo capacitation. The effect of nucleotides was reversed by A1 antagonists. Finally the effect of cAMP, ADO and both molecules in NCC was inhibited with PLC and PKA inhibitors, suggesting that these two transduction pathways may be involved in nucleotides-induced capacitation in bovines.