LPA regulates first trimester trophoblast functions

BELTRAME JS; SORDELLI MS; FRANCHI AM; RIBEIRO ML

Chascomus

Congreso; XVI Jornadas de la Sociedad Argentina de Biología, Chascomús, 1-3 diciembre 2014.; 2014

Lysophosphatidic acid (LPA) is a small and bioactive phospholipid that plays an important role during the window of implantation. Previously, we observed that LPA, through LPA3 receptor, acts like a pro-implantatory molecule in the uterus. The aim of the present study was to investigate the effect of LPA in the fetal side of the maternal-fetal interphase. Thus, we studied LPA role in first trimester trophoblast vascularization and proliferation. First, we detected the expression of the mRNA and the protein of LPA3 receptor, as well as lyso-PLD, the main enzyme that synthesizes LPA, in first trimester trophoblast cell line, HTR-8/SVneo. Also, LPA3 was localized in the cytoplasm and plasma membrane of HTR-8/SVneo cells. Then, HTR-8/SVneo cells were incubated with LPA to assay tube formation (5, 10 and 20 μM, 3 and 6 hs) and proliferation (50 μM, 48 hs). After 3 hs of incubation, LPA stimulated capillary tube formation at the three concentrations tested (p