Alterarion of liver lipid metabolism by prenatal hyperandrogenism

GISELLE ADRIANA ABRUZZESE; MARIA FLORENCIA HEBER; LEANDRO MARTIN VELEZ; ALICIA BEATRIZ MOTTA

Chascomus

Congreso; XVI Jornadas Anuales de la SAB; 2014

SAB

ALTERATION OF LIVER LIPID METABOLISM BY PRENATAL HIPERANDROGENISM Abruzzese GA, Heber MF, Vélez LM, Motta AB. CEFYBO-UBA-CONICET.(giselleabruzzese@gmail.com) Polycystic ovarian syndrome (PCOS) is associated to Metabolic Syndrome (MetS), which could cause liver injury and even lead to non-alcoholic fatty liver disease (NAFLD) that correlates to altered lipid metabolism. In our laboratory we have developed a murine PCOS model by prenatal hyperandrogenism in which we demonstrated elevated risk of MetS and an incipient liver injury. Our objective is to evaluate the content of liver triglycerides (TG), and the peroxisome proliferator-activated receptor (PPAR) gamma system and its co-activator PGC1-alfa, both involved in lipid metabolism. Pregnant Sprague Dawley rats were separated in two groups: control (C) and prenatal hiperandrogenizated (PH) with testosterone. In puberal offspring, we evaluated mRNA and protein levels of the PPAR gamma and PGC1-alfa system by qRT-PCR and Western blotting, respectively. We found that liver TG content and mRNA levels of PPAR gamma corresponding to C and PH were similar whereas protein levels of PPAR gamma were higher in PH than C group and mRNA expression of PGC-1 was decreased in PH compared to C group. We conclude that the molecular pathway controlling hepatic lipid metabolism, mediated by these molecules, is affected in the PH group.