- Participation of LPA3 receptor in the decidualization and vascularization processes in the rat uterus.

BELTRAME JS; SORDELLI MS; DYMITRENKO A; CELLA M; SALES ME; BLOIS S; FRANCHI AM; RIBEIRO ML

Foz de Iguazú

Simposio; V Latin American Symposium on Maternal Fetal Interaction & Placenta (SLIMP) and IV Latin American Symposium on Reproductive Immunology (LASRI); 2013

Latin American Symposium on Maternal Fetal Interaction & Placenta (SLIMP) and Latin American Reproductive Immunology Society

Background: Lysophosphatidic acid (LPA) plays a crucial role in pregnancy establishment. Knock out of LPA3, an LPA receptor, have implantation deficiencies. We investigated the participation of LPA3 during decidualization and vascularization. Methods: Uterine slices of Wistar rats in day 5 of gestation were incubated with LPA and DGPP (LPA3 receptor antagonist). IGFBP-1 and IL-10 expression was determined. Also, females in day 5 of gestation received an intra-uterine dosis of DGPP. Decidualization and vascularization characteristics were analyzed on day 8 of gestation. Results: LPA, through its LPA3 receptor, augmented the expression of IGFBP-1 and IL-10. The in vivo treatment produced an increase in the embryonic resorption percentage, alterations in the deciduas morfological structure, signs of cellular death, leucocytes infiltration and reduced the transversal section of two main vessels that irrigate the uterus. Conclusions: The results suggest that LPA3 receptor participates in the decidualization and vascularization essential for embryo implantation.