Serine antiprotease Trappin-2 induces IL-9 in human peripheral blood mononuclear cells

ALIJA, A; AMBROSI, N; HERNANDEZ DEL PINO, R; GUERRIERI, D; ALVAREZ, I; TATEOSIAN, NANCY; GARCIA, V; CHULUYAN, E

hawaii

Congreso; American Assosiation of Immunologists. 100th Annual Meeting of the American Association of Immunologists.; 2013

Trappin-2 is a 9.9 kDa serine protease inhibitor produced by epithelial cells and immune cells such as macrophages and ãäT cells. It has anti-inflammatory and microbicide activities. The aim of the present work was to evaluate the effect of Trappin-2 on peripheral blood mononuclear cells (PBMC) by analyzing proliferation response, apoptosis, cytokine production and transcription factors expression. After 5 days of culture, Trappin-2 decreased lymphocyte proliferation induced by Mtb-antigen and diminished the early apoptosis of cells. Moreover, culture supernatants derived from PBMCs treated with Trappin-2 (48 h) showed a slight increase in IL-4 production, what was confirmed by intracellular staining of IL-4 on CD3 T cells. Besides, Trappin-2 significantly increased GATA-3 expression on PBMCs as determined by Western Blot. However, the most significant result was a clear increase in IL-9 expression on CD4 T cells, after 48 hours of culture of cells with Trappin-2. Moreover, the expression of PU.1 was also increased on Trappin-2-treated cells. Finally, TGF-â was measured in the culture supernatants of PBMC treated withTrapin-2, given that naive CD4+ T cells differentiate in vitro into IL-9-secreting cells by effect of IL-4 and TGF-â. The results obtained showed that Trappin-2 in fact increased TGF-â at 48 h of culture. Overall, our findings suggest that Trappin-2 induces a cytokine microenvironment compatible with a potential Th9 pattern.