Nitric oxide and peroxynitrite secretion from lipopolysaccharide (LPS) treated peripheral blood mononuclear cell (PBMC) inhibits decidual anandamine (AEA) degradation.

WOLFSON ML; AISEMBERG J; VERCELLI CA; FRANCHI AM

Foz de Iguaçu, Parana

Simposio; V Latin American Symposium on Maternal Fetal Interaction & Placenta and IV Latin American Chapter of the American Society for Reproductive Immunology; 2013

The major endocannabinoid anandamide, when in excess, is associated to pregnancy loss. Using an LPS-induced embryonic resorption (EmR) model, we studied the interaction of the infiltrating immune cells and the decidual endocannabinoid system using a transwell system. We observed a decreased in FAAH activity (the main AEA degradating enzyme) in the decidua exposed to PBMCs from LPS-treated mice. We have previously shown that nitric oxide (NO) has role in EmR. Therefore we analysed whether this molecule participated in the modulation of decidual FAAH activity. When animals were treated with LPS in the presence of an inhibitor of iNOS (aminoguanidine), LPS effects were abrogated. Next, we used a peroxynitrite scavenger (quercetine) in the decidua chamber, observing a reversal of LPS effects. Moreover, LPS induced an increase in protein nitration in decidua, effect that was reversed by aminoguanidine treatment. These results suggest that PBMC soluble factors could be involved in LPS-induced EmR.