Cannabinoid Receptor Activation Correlates with the Proapoptotic Action of LPS in a murine model of embryonic resorption.

SALAZAR, AI; DOMÍNGUEZ RUBIO, AP; DAVIO, CA; FRANCHI, AM

Foz de Iguazú

Congreso; V Latin American Symposium on Maternal Fetal Interaction & Placenta and IV Latin American Chapter of the American Society for Reproductive Immunology; 2013

Latinamerican Society for Reproductive Immunology

We have demonstrated that the endocannabiods mediate the deleterious effect of LPS on the uterine tissue. In this study we describe the apoptotic effect of LPS and the endocannabinoid anandaminde (AEA) in murine uterine fragments in the 7th day of pregnancy by analyzing their nucleus structure and measuring caspase activity. The apoptotic effect of LPS is reverted with the co incubation of cannabinoid receptors type 1 and 2 antagonists. We also studied the underlying signal transduction pathway that could be involved. We measured the cyclic AMP levels in presence of LPS and different concentrations of AEA. Both molecules produced a decreased in cyclic AMP, indicating that receptors are coupled to Gi protein. These results show that both LPS and AEA produce tissue damage and apoptosis, and that the endocannabinoid receptors could participate in the LPS mechanism of action. We also postulate the cyclic AMP pathway as responsible for this mechanism.