CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
HYPOXIA INDUCIBLE FACTOR (HIF) MODULATES ENDOCANNABINOID SYSTEM AND NOS ACTIVITY IN HUMAN PLACENTA
Autor/es:
ABAN C., LEGUIZAMÓN G., CELLA M., GARBAGNATI N., RAMIL V., FRANCHI A., FARINA MG.
Lugar:
Foz do Iguazu
Reunión:
Congreso; V SLIMP Latin American Symposium on Maternal Fetal Interaction & Placenta - IV LASRI Latin American Symposiumon Reproductive Immunology; 2013
Resumen:
Endocannabinoids are lipid mediators that could regulate NOS activity in human placenta. Besides, Hypoxia-inducible Factor (HIF) is a transcription factor that plays an important role in placental physiology. In this study, we investigate the effect of HIF on the endocannabinoid system and NOS activity in human placenta. Methods: Explants obtained from normal placentas were cultured with cobalt chloride (that stabilizes HIF) and/or AM251, cannabinoid receptor antagonist. Expression of the endocannabinoid system was analyzed (RT-PCR and Western Blot). NOS activity was quantified by Bredt & Snyder method. Results: HIF increase NAPE-PLD and decrease FAAH expression, enzymes involved in the main pathways of synthesis and degradation of endocannabinoids. Furthermore, variations in endocannabinoids levels generated by HIF stimulates NOS activity and co-incubation with AM251 revert this effect. Conclusions: HIF modulate the expression of some components of endocannabinoid system suggesting that variations in the endocannabinoid levels could regulate NOS activity in human placenta.