CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Effect of enriched environment housing on glutamate-induced damage in adult rat retina
Autor/es:
DORFMAN D; DIEGO FERNANDEZ; CHIANELLI, MONICA; ARANDA M; ROSENSTEIN R
Lugar:
Seattle
Reunión:
Congreso; The Association For Research In Vision And Ophthalmology (Arvo); 2013
Institución organizadora:
ARVO
Resumen:
Abstract
Purpose:Glutamate, the main excitatory
neurotransmitter in the retina, is toxic when present in excessive amounts. In
fact, glutamate-induced excitotoxicity has been involved in different retinal
diseases. The aim of this study was to elucidate whether enriched environment
(EE) housing protects the retina from glutamate-induced damage in adult rats.
Methods:Adult male Wistar rats were
intravitreally injected with a 4 μL of 0.3 M
glutamate in one eye and vehicle in the contralateral eye. Immediately after
injections, animals were housed in standard laboratory cages (standard
environment, (SE)) or EE. EE consisted of big cages containing 6 animals/cage,
and several food hoppers, wheels and different objects repositioned once/day
and fully substituted once/week. After 1 week of SE or EE housing, retinal
function (scotopic electroretinography, ERG), and retinal morphology (optical
microscopy) were analyzed. Müller cell glial fibrillary acidic protein (GFAP)
levels and Brn3a(+) retinal ganglion cell (RGC) number were assessed by
immunohistochemistry. Anterograde transport from the retina to the superior
colliculus was examined after an intravitreal injection of cholera toxin β-subunit.
Results:In SE-housed (control) animals,
glutamate induced a significant decrease in ERG a- and b- wave and oscillatory
potential amplitude, whereas EE housing significantly reduced these
alterations. In animals exposed to SE, glutamate induced a significant RCG
loss, and an increase in Müller cell GFAP levels, which were reversed by EE
housing. In control animals, glutamate induced a deficit in the anterograde
transport which was preserved by EE housing.
Conclusions:These results suggest that EE housing
could become a future and novel therapy for retinal diseases which involve
glutamate-mediated retinal damage.