Antioxidant and scavenger properties of 6 PP, a prenylated flavonoid isolated from Dalea elegans

ELINGOLD I, ISOLABELLA MP, CASANOVA MB, CELENTANO AM, PEREZ C, DIEZ R, CABRERA JL, DUBIN M

Buenos Aires

Jornada; VIII Jornadas Multidisciplinarias de la Sociedad Argentina de Biología; 2006

Sociedad Argentina de Biología

ROPERTIES OF 6PP, A PRENYLATED     FLAVONOID ISOLATED FROM Dalea elegans.   Elingold I1, Isolabella MP2, Casanova MB1, Celentano AM3, Pérez C4, Diez RA2,  Cabrera JL5, Dubin M1.   1CEFYBO UBA-CONICET, Dptos. 2Farmacología y 3Microbiología, Fac. Medicina, 4Cát.  Farmacología, Fac. Odontología (UBA) y 5Farmacognosia, Fac. Cs. Qcas. (UNC). E-mail: rey-elrond@ciudad.com.ar.     The polyphenolic components of higher plants may act as free radical scavengers  or through other mechanisms contributing to anticarcinogenic or cardioprotective action.  The prenylated flavonone 2´-4´-dihidroxy-5´-(1´´´-dimethylallyl)-6-prenylpinocembrin (6PP) was isolated from the roots of the Argentinian plant Dalea elegans. Previous results  showed activity against oxacillin-sensitive and resistant Staphylococcus aureus. The aim of our work was to evaluate  6PP antioxidant properties. Enzymatic lipid peroxidation  in rat liver microsomes was determined by measuring the in vitro formation of thiobarbituric acid-reactive substances (TBARS).  6PP significantly inhibited malondialdehyde production in a concentration dependent-manner between 6.25 and 100 mM, (87.9±4.3-%-100 mM, p< 0.01). Microsomal oxygen uptake was determined by  the polarographic method, employing an oxygraph with a Clark electrode. 6PP addition inhibited uptake oxygen between 25 and 100 mM, p<0.01. The ability of scavenging free radicals was measured by DPPH reduction spectrophotometric assay, at 517 nm. 6PP exhibited significant scavenging activity in a concentration-dependent manner between 10 and 100 mM, (63±9%-100 mM, p<0.001). The antioxidant capacity of 6PP was quantitated spectrophotometrically through the formation of a phosphomolibdenum complex at 695 nm. 6PP was able to significantly reduce Mo (VI) to Mo (V) at 50 and 100 mM, p<0.05. Our preliminary results demonstrate that 6PP exerts both antioxidant and antiradical activities. Further studies are needed to evaluate possible therapeutic applications.