CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Antioxidant and scavenger properties of 6 PP, a prenylated flavonoid isolated from Dalea elegans
Autor/es:
ELINGOLD I, ISOLABELLA MP, CASANOVA MB, CELENTANO AM, PEREZ C, DIEZ R, CABRERA JL, DUBIN M
Lugar:
Buenos Aires
Reunión:
Jornada; VIII Jornadas Multidisciplinarias de la Sociedad Argentina de Biología; 2006
Institución organizadora:
Sociedad Argentina de Biología
Resumen:
ROPERTIES OF 6PP, A PRENYLATED
FLAVONOID ISOLATED FROM Dalea elegans.
Elingold I1, Isolabella MP2, Casanova MB1, Celentano AM3, Pérez C4, Diez RA2, Cabrera JL5, Dubin M1.
1CEFYBO UBA-CONICET, Dptos. 2Farmacología y 3Microbiología, Fac.
Medicina, 4Cát.
Farmacología, Fac. Odontología (UBA) y 5Farmacognosia, Fac. Cs. Qcas. (UNC). E-mail: rey-elrond@ciudad.com.ar.
The polyphenolic components of higher plants may act as free radical
scavengers or through other mechanisms contributing
to anticarcinogenic or cardioprotective action. The prenylated flavonone
2´-4´-dihidroxy-5´-(1´´´-dimethylallyl)-6-prenylpinocembrin (6PP) was isolated
from the roots of the Argentinian plant Dalea elegans. Previous results showed activity against oxacillin-sensitive
and resistant Staphylococcus aureus. The aim of our work was to evaluate 6PP antioxidant properties. Enzymatic lipid
peroxidation in rat liver microsomes
was determined by measuring the in vitro formation of thiobarbituric acid-reactive
substances (TBARS). 6PP significantly
inhibited malondialdehyde production in a concentration dependent-manner
between 6.25 and 100 mM, (87.9±4.3-%-100 mM, p<
0.01). Microsomal oxygen uptake was determined by the polarographic method, employing an oxygraph with a Clark electrode. 6PP addition
inhibited uptake oxygen between 25 and 100 mM, p<0.01.
The ability of scavenging free radicals was measured by DPPH reduction
spectrophotometric assay, at 517 nm. 6PP exhibited significant scavenging
activity in a concentration-dependent manner between 10 and 100 mM, (63±9%-100 mM, p<0.001). The antioxidant capacity of 6PP
was quantitated spectrophotometrically through the formation of a
phosphomolibdenum complex at 695 nm. 6PP was able to significantly reduce Mo
(VI) to Mo (V) at 50 and 100 mM, p<0.05. Our preliminary results demonstrate
that 6PP exerts both antioxidant and antiradical activities. Further studies
are needed to evaluate possible therapeutic applications.