CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Hormonal regulation of AQP9 molecular expression in syncytiotrophoblast of human term placenta
Autor/es:
MARINO GI; FARINA M; NOVAK M; IBARRA C; DAMIANO AE
Lugar:
Rosario, Santa Fe
Reunión:
Congreso; XLII Reunión Anual Sociedad Argentina de Investigación en Bioquímica y Biología Molecular; 2006
Institución organizadora:
Sociedad Arentina de Investigación en Bioquímica y Biología Molecular
Resumen:
The syncytiotrophoblast of human term placenta (hST) is not only involved in the transport of water and solutes between mother and fetus but it also produces the majority of the placental hormones throughout pregnancy. Because of its syncytial characteristics the mechanisms of transport should take place primarily via transcellular routes. Previously, we have reported the expression and localization of AQP3, AQP7 and AQP9 in hST. In pathological conditions like preeclamptic pregnancies, we have also observed an over expression of AQP9 correlating with an increase of human chorionic gonadotropin hormone (hCG). The aim of this study is to determine if hCG or steroid hormones as progesterone (P4) may regulate AQP9 molecular expression. Normal placental explants were cultured and treated with P4 or hCG.  Semiquantitative RT-PCR and Western blot and Immunohistochemistry were performed to evaluate AQP9 expression. After P4 treatment, AQP9 protein expression decreased and it is localized in the apical membrane of hST. On the other hand, after hCG treatment we showed that AQP9 levels were not modified but it is also localized in the cytoplasm region. Our results suggest that P4 and hCG may be involved in the regulation of AQP9 expression in hST. However, much further work is needed to clarify its role in human placenta.