CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
ROLE OF CGRP AND PROSTACYCLIN IN THE SEX-LINKED DIFFERENCES OF THE RELAXANT EFFECTS OF ANANDAMIDE IN RAT MESENTERIC ARTERIES
Autor/es:
ROXANA PERONI; TAMARA ABRAMOFF; MARÍA LAURA RIBEIRO; ANA MARÍA FRANCHI; EDA ADLER-GRASCHINSKY
Lugar:
Córdoba, Argentina
Reunión:
Congreso; XXXVIII Reunión Anual de la Sociedad Argentina de Farmacología Experimental; 2006
Institución organizadora:
Sociedad Argentina de Farmacología Experimental (SAFE)
Resumen:
The aim of this work was to study the mechanisms involved in the greater relaxations caused by anandamide (AEA) in mesenteric beds isolated from female compared to male rats (Peroni et al., Eur. J. Pharmacol. 493:151, 2004). The AEA-induced relaxations were abolished by the nitric oxide synthase inhibitor 100 mM L-NAME in intact as well as in de-endothelized male and female mesenteric beds. Sensory in vivo denervation also markedly reduced the relaxation caused by AEA in either male or female mesenteries. On the other hand, the remotion of the endothelium enhanced the relaxations caused by AEA in mesenteries isolated from male and ovariectomized female but not from sham-operated female rats. The calcitonin gene-related peptide (CGRP) content in rat mesenteric beds was higher in female than in male rats, faded by ovariectomy and restored to control values by chronic treatment with 17b-estradiol. This latter procedure also increased CGRP content in males up to the same levels observed in females. With regard to prostanoids, the ratio prostacyclin / thromboxane A2, that did not differ between male and female mesenteries under control conditions, it was reduced in males after exposure to AEA, due to the decrease in the prostacyclin tissue content. Moreover, the cyclooxygenase-2 inhibitor 0.1 mM NS-398 reduced the relaxations caused by AEA solely in female rats. It is proposed that relaxing factors such as CGRP and prostacyclin contribute to the higher relaxations caused by anandamide in the vasculature of female rats.