Effect of the lipopolysaccharide from E. Coli in the ovary in a rat model of subclinical infection

CAÑUMIL, VANESA A.; BELTRAME JS; DE LA CRUZ BORTHIRY, FERNANDA L.; ANA MARÍA FRANCHI; SCHEFFER, FRIDA; BOGETTI, MARÍA EUGENIA; RIBEIRO MARÍA L

Mar del Plata

Congreso; Reunión Conjunta SAIC-SAB-AAFE-AACYTAL 2023; 2023

SAIC-SAB-AAFE-AACYTAL

Subclinical infections cause dysregulation of immune homeostasis that could haveserious consequences for pregnancy. It has been postulated that alterations in theinflammatory response might cause placental dysfunction impacting progenydevelopment. Previous results from our laboratory show that the administration oflipopolysaccharide from Escherichia coli (LPS) as an infection stimulus to rats duringearly gestation is associated with a decrease in fertility as it affects the maternal-fetalinterface and the neurodevelopment of the offspring. The aim of this study was toelucidate if the effect of LPS was caused by a direct action on the uterus, orindirectly by a systemic contribution via the ovary. For this, pregnant rats of theWistar strain received vehicle (saline, control) or intraperitoneal LPS (20 μg/kg onday 6 + 50 μg/kg on days 7, 8 and 9 of gestation). The animals were euthanized onday 15 of gestation and the ovaries were surgically removed and stained withhematoxylin and eosin.The number and size of the corpora lutea, and the numberand type of luteal cells were quantified. Data were analyzed using one-way ANOVA.Differences were considered significant when p