Effect of maternal diets enriched in pufas administrated to diabetic rats during early postimplantation on structures involved in decidual histotrophic function and feto-placental growth

SABRINA LORENA ROBERTI, CINTIA ROMINA GATTI, MARÍA LAURA LEONARDI, ROMINA HIGA, ALICIA JAWERBAUM.

Virtual

Congreso; LXVI Reunión Anual de la Sociedad Argentina de Investigación Clínica (SAIC); 2021

Maternal diabetes affects pathways regulated by nutrients in the rat decidua, which may impact on the decidual histotrophic nutrition, involving uterine glands (UG) and the glycogenic area (GA). It is known that maternal diets enriched in polyunsaturated fatty acids (PUFAs) regulate these pathways at early postimplantation. Also, an adequate decidualization is required for feto-placental development and the uNK cells are involved in angiogenesis and vessels remodeling at placentation. uNK cells, involved in angiogenesis and vessels remodeling at placentation are also important for an adequate decidualization required for feto-placental development.Aim: To evaluate the effect of diets enriched in sunflower and chia oil (rich in n-6 and n-3 PUFAs respectively) administrated during early postimplantation to diabetic rats on PAS staining of the GA and UG and on the number of uNK cells at day 9 of pregnancy and on decidual, fetal and placental growth at day 14 of pregnancy.Methods: Pregestational diabetes was induced in Wistar rats by streptozotocin (50 mg/kg). On days 7 to 9 of pregnancy diabetic rats received a standard diet or diets enriched in 6% of sunflower or chia oil. On day 9 of pregnancy GA, UG and uNK cells in the decidua were evaluated by PAS staining. On day 14 of pregnancy the fetal cephalic length was measured and the decidua and placenta were weighted. Results: At day 9 of pregnancy PAS staining of GA (p