CEFYBO 02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
capítulos de libros
Título:
Sirtuins: Methods and Protocols
Autor/es:
LEI ZHONG; CARLOS SEBASTIÁN; BÁRBARA MARTÍNEZ-PASTOR; D.M. SILBERMAN; RAUL MOSTOSLAVSKY
Libro:
Methods in Molecular Biology
Editorial:
Springer Science+Business Media
Referencias:
Año: 2013; p. 149 - 163
Resumen:
Most of the
sirtuins? nuclear substrates identified so far are histones or other
chromatin-associated proteins and, thus, it is of special relevance the
development of good biochemical techniques to analyze the biology of these
proteins in the context of chromatin. Here, we describe several of the
chromatin-based techniques to identify sirtuins? substrates, including a
chromatin immunoprecipitation (ChIP) protocol, an acid- extraction protocol,
and a nucleosomal immunoprecipitation protocol to analyze putative sirtuin
chromatin interactors.
