CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Characterization of murine amniotic fluid B cells in normal pregnancy and in preterm birth
Autor/es:
JURIOL, LORENA; EHRHARDT, JENS; MALINOWSKY, KRISTIN; JENSEN, FEDERICO; BOMMER, IMKE; VALEFF, NATALIN; ZIEGLER, KATHARINA; ZYGMUNT, MAREK; MUZZIO, DAMIÁN; MATZNER, FRANZISKA; VENTIMIGLIA, MARÍA SILVIA
Revista:
REPRODUCTION
Editorial:
BIOSCIENTIFICA LTD
Referencias:
Año: 2019 vol. 158 p. 369 - 376
ISSN:
1470-1626
Resumen:
The amniotic fluid provides mechanical protection and immune defense against pathogens to the fetus. Indeed, components of the innate and adaptive immunity, including B cells, have been described in the amniotic fluid. However, limited information concerning phenotype and functionality of amniotic fluid B cells is available. Hence, we aimed to perform a full phenotypical and functional characterization of amniotic fluid B cells in normal pregnancy and in a mouse model of preterm birth. Phenotypic analysis depicted the presence of two populations of amniotic fluid B cells: an immature population, resembling B1 progenitor cells and a more mature population. Further isolation and in vitro co-culture with a bone marrow stroma cell line demonstrated the capacity of the immature B cells to mature. This was further supported by spontaneous production of IgM, a feature of the B1 B cell sub-population. An additional in vitro stimulation with lipopolysaccharide induced the activation of amniotic fluid B cells as well as the production of pro and anti-inflammatory cytokines. Furthermore, amniotic fluid B cells were expanded in the acute phase of LPS-induced preterm birth. Overall our data add new insight not only on the phenotype and developmental stage of the amniotic fluid B1 B cells but especially on their functionality. This provides important information for a better understanding of their role within the amniotic fluid as immunological protective barrier, especially with regard to intraamniotic infection and preterm birth.