CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Oligodendrogenesis in iron-deficient rats: effect of apotransferrin.
Autor/es:
ROSATO-SIRI MV; BADARACCO ME; ORTIZ EH; BELFORTE N; CLAUSI MG; SOTO EF; BERNABEU R; PASQUINI JM
Revista:
JOURNAL OF NEUROSCIENCE RESEARCH
Editorial:
WILEY-LISS, DIV JOHN WILEY & SONS INC
Referencias:
Lugar: United States; Año: 2010 vol. 88 p. 1695 - 1707
ISSN:
0360-4012
Resumen:
In rats, iron deficiency produces an alteration in myelin formation.
However, there is limited information on the effects of this condition
on oligodendroglial cell (OLGc) proliferation and maturation. In the
present study, we further analyzed the hypomyelination associated with
iron deficiency by studying the dynamics of oligodendrogenesis. Rats
were fed control (40 mg Fe/kg) or iron-deficient (4 mg Fe/kg) diets from
gestation day 5 until postnatal day 3 (P3) or 11 (P11). OLGc
proliferation, migration and differentiation were investigated before
and after an intracranial injection of apotransferrin at 3 days of age
(P3). The proliferating cell population was evaluated at P3.
Iron-deficient (ID) animals showed an increase in the oligodendrocyte
precursors cell (OPC) population in comparison with controls. The
overall pattern of migration of cells labeled with BrdU was investigated
at P11. Iron deficiency increased the amount of BrdU+ cells
in the corpus callosum (CC) and decreased OLGc maturation and myelin
formation. Changes in nerve conduction were analyzed by measuring visual
evoked potentials. Latency and amplitude were significantly disturbed
in ID rats compared with controls. Both parameters were substantially
normalized when animals were treated with a single intracranial
injection of 350 ng apotransferrin (aTf). The current results give
support to the idea that iron deficiency increases the number of
proliferating and undifferentiated cells in the CC compared with the
control. Treatment with aTf almost completely reverted the effects of
iron deficiency, both changing the migration pattern and increasing the
number of mature cells in the CC and myelin formation.