Neutrophil elastase treated-dendritic cells induce CD4+FOXP3+ T cells in vitro

NICOLAS O AMIANO; NANCY L TATEOSIAN; DIEGO GUERRIERI; MERCEDES L SÁNCHEZ; MARIA J COSTA; ROMINA M REITERI; XIMENA VILLALONGA; PAULO CESAR MAFFIA

HUMAN IMMUNOLOGY

Año: 2009

0198-8859

We have previously shown that neutrophilic elastase converts human immature dendriticcells (DCs) into TGF-b-secreting cells and reduces its allostimulatory ability. Since TGF-b has beeninvolved in Treg induction we analyzed whether elastase or neutrophil-derived culture supernatanttreatedDCs induce CD4+FOXP3+ cells in a mixed lymphocyte reaction (MLR). We found that elastaseor neutrophil-derived culture supernatant (PMNs-CS) treated-DCs increased TGF-b and decreased IL-6production. We also observed a higher number of CD4+FOXP3+ cells in the MLR cultures induced byelastase or PMNs-CS -treated CDs but not with untreated DCs. The higher number of CD4+FOXP3+ Tcell population was not observed when the enzymatic activity of elastase was inhibited with anelastase specific inhibitor or when a TGF-β blocking antibody was added during the MLR. These datasuggest that tolerigenic DCs generated by elastase could be relevant in the pathogenesis of severaldiseases where the inflammatory infiltrate contains high numbers of neutrophils and high elastaseconcentrations.