CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Treatment with melatonin after onset of experimental uveitis attenuates ocular inflammation
Autor/es:
PH SANDE; D DORFMAN ; FERNANDEZ DC; M.CHIANELLI; DOMÍNGUEZ RUBIO AP; FRANCHI AM; DM SILBERMAN; ROSENSTEIN RE; DA SAENZ
Revista:
BRITISH JOURNAL OF PHARMACOLOGY
Editorial:
WILEY-BLACKWELL PUBLISHING, INC
Referencias:
Lugar: Londres; Año: 2014 vol. 171 p. 5696 - 5707
ISSN:
0007-1188
Resumen:
Background and purpose: Uveitis is a prevalent intraocular inflammatory disease and one of the most damaging ocular conditions. Previously, we showed that melatonin prevents ocular inflammation induced by an intravitreal injection of bacterial lipopolysaccharide (LPS) in the Syrian hamster. With this background, the aim of this work was to assess the anti-inflammatory effect of melatonin administered after the onset of ocular inflammation. Experimental approach: Eyes were intravitreally injected with vehicle or LPS. Melatonin was intraperitoneally supplied every 24 h, starting 12 h or 24 h post-LPS injection. A clinical evaluation (with a score index based on clinical symptoms), the number of infiltrating cells, protein concentration, and prostaglandin (PG) E2 and PGF2 levels in the aqueous humor, as well as retinal nitric oxide synthase (NOS) activity, lipid peroxidation and TNFα levels were assessed. Retinal function was assessed by scotopic electroretinography, and light microscopy and immunohistochemistry were used to evaluate the retinal structure. Key results: Both post-treatments with melatonin significantly decreased clinical symptoms, reduced the leakage of cells and proteins, and decreased PG levels in aqueous humor from eyes injected with LPS. In addition, melatonin avoided the decrease in scotopic electroretinogram aand b-wave amplitude, protected the retinal structure, and reduced the increase in NOS activity lipid peroxidation, and TNFα levels induced by LPS. Conclusions and Implications: These results indicate that a post-treatment with melatonin significantly attenuated ocular inflammation induced by LPS in the Syrian hamster, and support the use of melatonin as a therapeutic resource for uveitis treatment.