CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Saturated fat-rich diet increases fetal lipids and modulates LPL and leptin receptor expression in rat placentas.
Autor/es:
MAZZUCCO MB; HIGA R; CAPOBIANCO E; KURTZ M; JAWERBAUM A; WHITE V
Revista:
JOURNAL OF ENDOCRINOLOGY
Editorial:
BIOSCIENTIFICA LTD
Referencias:
Lugar: Bristol; Año: 2013 vol. 217 p. 303 - 315
ISSN:
0022-0795
Resumen:
Metabolic alterations in obese and overweight mothers impact the placenta and the fetus, leading to anomalies in fetal growth and lipid accretion. The primary aim of the study was to examine the effect of a saturated fat-rich diet (FD) on growth, lipid accretion, and lipases, leptin and leptin receptor (ObR) expression in the placenta and fetal liver. We also aimed to find a role for fetal leptin in the modulation of placental and fetal liver lipase and ObR expression. Six-week-old rats were fed with a standard rat chow (control) or a 25% FD for 7 weeks until mating and during pregnancy. Also, in a group of control rats, fetuses were injected with leptin on days 19, 20, and 21 of pregnancy. On day 21, we assessed lipidemia, insulinemia, and leptinemia in mothers and fetuses. In the placenta and fetal liver, lipid concentration was assessed by thin layer chromatography (TLC) and the gene expression of lipoprotein lipase (LPL), endothelial lipase, insulin receptor (Insr), leptin, and ObR by RT-PCR. The FD induced hypertriglyceridemia and hyperleptinemia (P lower than 0.01) in mothers and fetuses, an increase in maternal (P lower than 0.05) and fetal weight (P lower than 0.01), overaccumulation of lipids in fetal liver (P lower than 0.01), and enhanced leptin expression in the placenta and fetal liver ( P lower than 0.05). Placental expression of IR and LPL was increased (P lower than 0.05), and ObR decreased (P lower than 0.05) in the FD group. Fetal administration of leptin induced the placental and fetal liver downregulation of ObR (P lower than 0.05) and upregulation of LPL expression (P lower than 0.05). The FD led to increased fetal lipid levels, which may result from high maternal lipid availability and fetal leptin effects.