CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Reduced methacholine-induced submandibular salivary secretion in rats with experimental periodontitis
Autor/es:
AMER M; ELVERDIN JC; FERNÁNDEZ-SOLARI J; MEDINA VA; CHIARENZA AP; VACAS MI
Revista:
ARCHIVES OF ORAL BIOLOGY
Editorial:
PERGAMON-ELSEVIER SCIENCE LTD
Referencias:
Año: 2011 vol. 56 p. 421 - 427
ISSN:
0003-9969
Resumen:
Abstract: Objective: Saliva is the first barrier to the entry of bacteria and viruses into the body and is considered a necessary instrument in oral health. Intraperitoneal injection of lipopolysaccharide endotoxins results in submandibular gland (SMG) hyposalivation. The objective of present studies was to assess if periodontitis, a chronic inflammatory disease caused by oral bacteria, alters cholinergicinduced SMG salivary secretion. Design: An experimental periodontitis model (EP) (cotton thread ligature around the neck of the first lower molars) was used. Male Wistar rats (300-380g) were randomly divided into 3 groups: control, 7 days-bilateral EP and 7 days-unilateral EP (to study if there were different effects at the ipsilateral and contralateral side). The following determinations were performed in SMG: 1) dose response curves to the cholinergic agonist methacholine, 2) prostaglandin E (PGE) content, 3) inducible nitric oxide synthase (iNOS) activity and 4) histology of gland sections. Results: The molars with EP, no matter the group, exhibited significant and similar bone loss (p<0.001). Bilateral EP reduced methacholine-induced salivary secretion (p<0.05, dose 1 ìg/kg; p<0.001, dose 3-30 ìg/kg), increased PGE content (p<0.01), stimulated iNOS activity (p<0.05). Ipsilateral glands of unilateral EP animals presented lower methacholine-induced salivary secretion (p<0.05, dose 3 ìg/kg; p<0.001, dose 10-30 ìg/kg), and higher PGE content than contralaterals (p<0.001). In turn, at 3 and 10 ìg/kg of methacholine, contralateral glands showed significantly lower  secretion than control animals (p<0.001). Histological studies of glands revealed partial loss of secretor granular material and periductal oedema in the bilateral and unilateral EP groups as compared to controls. Conclusions: As far as we know, the present results demonstrate for the first time that EP reduces methacholine-induced SMG salivary secretion.