CEFYBO   02669
CENTRO DE ESTUDIOS FARMACOLOGICOS Y BOTANICOS
Unidad Ejecutora - UE
artículos
Título:
Nitric oxide synthase 1 and cyclooxygenase-2 enzymes are targets of muscarinic activation in normal and inflamed NIH3T3 cells
Autor/es:
ESPAÑOL, ALEJANDRO JAVIER; GOREN NORA; RIBEIRO MARÍA; SALES MARÍA ELENA
Revista:
INFLAMMATION RESEARCH
Editorial:
Birkhäuser
Referencias:
Lugar: Switzerland; Año: 2010 vol. 59 p. 227 - 238
ISSN:
1023-3830
Resumen:
Objective: Fibroblasts are sentinel cells that could intermediate the immune reaction ininflammatory process. In this work, we investigate the action of the muscarinic agonistcarbachol (CARB) on the expression and function of nitric oxide synthase (NOS) andcyclooxygenase (COX) in fibroblasts under normal or inflammatory conditions. Methods:the normal fibroblast cell line, 3T3, from NIH swiss mouse embryo, was used.Inflammatory millieu was mimicked with lypopolisaccharide (LPS) (10 ng/ml) plusinterferon gamma (IFNγ) (0.5 ng/ml). Nitric oxide (NO) and prostaglandin E2 (PGE2)production were measured by Griess reagent and radioimmunoassay respectively. NOS,COX and nuclear transcription factor kappa B (NF-κB) were studied by Western blot.Results: CARB increased NO synthesis by 57+/−5%, while a 14+/−310% increment in NOliberation was triggered by LPS plus IFNγ treatment. CARB added to LPS plus IFNγpotentated NO synthesis by 22+/−819%. CARB, also, up-regulated NOS1 proteinexpression via NF-κB activation. In addition CARB or LPS plus IFNγ stimulated PGE2synthesis by 72+/−8% and 42+/−5% respectively while, CARB added to LPS plus IFNγtreated cells, produced a sinergism in PGE2 liberation (130+/−12%) via COX-2.Conclusion: activation of mAChR can mimic mild inflammatory conditions or deepenspre-existing inflammation exerting a fine set-up on fibroblasts that in turn could be alertingimmune system.