Beneficial activity of Enterococcus faecalis CECT7121 in the anti-lymphoma

CASTRO, MARISA; MOLINA, MATÍAS; DI SCIULLO, MARÍA PAULA; AZPIROZ MARÍA; LEOCATA NIETO, FRANCISCO; STERÍN-SPEZIALE, NORMA; MONGINI, CLAUDIA; MANGHI, MARCELA

JOURNAL OF APPLIED MICROBIOLOGY

WILEY-BLACKWELL PUBLISHING, INC

Lugar: Nueva York; Año: 2010 vol. 109 p. 1234 - 1243

1364-5072

AIMS: To study the anti-tumour effects of Enterococcus faecalis CECT7121 on LBCcells, an aggressive murine T-cell lymphoma that kills the host in 18 days whenis intraperitoneally (i.p.) administrated.METHODS AND RESULTS: In vitro studies have shown that LBC cell proliferation was inhibited by Ent. faecalis CECT7121 stimulus in a dose-dependent manner, inducingapoptosis. The production of ceramide was involved in the latter effect. Toundertake in vivo studies, syngeneic BALB/c mice pre-treated i.p. with Ent.faecalis CECT7121 (2.5 ~ 10(8 ) CFU) were challenged i.p. with LBC cells (1.0 ~10(6) cells) the day after. On day 30 post-inoculation of LBC cells, 70% of Ent. faecalis CECT7121 pre-treated mice survived, whereas no survivals were recordedin the control group. A group of surviving mice was re-challenged with LBC cells,and 89% of them survived. Upon stimulation with irradiated LBC cells, spleen cellproliferation, high IFNƒÁ, IL-12 and IL-10 levels were observed in survivinganimals.CONCLUSIONS: Enterococcus faecalis CECT7121 affected multiple factors of thetumour establishment by the following methods: down-regulating the LBC cellproliferation and inducing apoptosis in these cells; and enhancing the immuneresponse that protects animals from lymphoma challenge and re-challenge.SIGNIFICANCE AND IMPACT OF THE STUDY: This study demonstrate that Ent. faecalisCECT7121 has potential as a probiotic that could facilitate the development ofnovel complements to therapeutic strategies against oncological diseases.