IFEVA   02662
INSTITUTO DE INVESTIGACIONES FISIOLOGICAS Y ECOLOGICAS VINCULADAS A LA AGRICULTURA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
A role for AtPRMT5 in the Arabidopsis circadian network
Autor/es:
SABRINA E. SANCHEZ; EZEQUIEL PETRILLO; ESTEBAN J. BECKWITH; C. ESTEBAN HERNANDO; MATIAS L. RUGNONE; XU ZHANG; JUSTIN O. BOREVITZ; CRAIG SIMPSON; JOHN BROWN; PABLO D. CERDÁN; M. FERNANDA CERIANI; PALOMA MAS; ALBERTO R. KORNBLIHTT; MARCELO J. YANOVSKY
Lugar:
Montreal, Canada
Reunión:
Congreso; Plant Biology 2010; 2010
Institución organizadora:
American Society of Plant Biologists
Resumen:
Circadian clocks allow plants to adjust multiple physiological and developmental processes in anticipation of daily and seasonal changes in the environment. A feedback loop composed of cycling gene products that control their own synthesis has been suggested as the central oscillatory mechanism. The clock is synchronized on a daily basis by light cycles perceived by phytochromes and cryptochromes, but the signalling pathway connecting these photoreceptors to the clock is largely ignored. We have conducted a genetic screen using Arabidopsis thaliana as a model system to identify novel components of the circadian signalling network, and identified a role for ATPRMT5 (Arabidopsis thaliana PROTEIN ARGININE METHYLTRANSFERASE 5) in the circadian system of Arabidopsis. Here, we found that the circadian period of the rhythm of leaf movement in prmt5 mutants is lengthened compared to that of wild type plants. Similar results were obtained for the circadian period of TOC1::LUC and CAB::LUC bioluminescence. The atprmt5 mutant has also alterations in the pattern of expression of clock associated genes such as CCA1, TOC1, LHY, PRR7 and PRR9. The flc mutation suppresses the late flowering phenotype of prmt5 mutants, but has no effect on its circadian phenotype. All this suggests that AtPRMT5 is involved in the regulation of several physiological responses through different molecular mechanisms. This is in agreement with the observation that PRMT5 methylates histone as well as non-histone proteins in many eukaryotic organisms. Possible mechanisms underlying the circadian phenotype of the mutant through genomic, molecular and genetic analysis will be discussed.