IFEVA   02662
INSTITUTO DE INVESTIGACIONES FISIOLOGICAS Y ECOLOGICAS VINCULADAS A LA AGRICULTURA
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Direct effect of ozone on cereal aphids.
Autor/es:
TELESNICKI M; MARTINEZ-GHERSA, M.A.; GHERSA CM
Lugar:
Cordoba
Reunión:
Congreso; Second Meeting Latin American Association of Chemical Ecology.; 2012
Institución organizadora:
Latin American Association of Chemical Ecology
Resumen:
Our understanding of ozone pollution effects on plant-insect-interaction is limited to the separate response of each organism to the pollutant. On one hand, ozone effect on plants is highly dependant on plant developmental age and exposure conditions. On the other hand, both positive and negative effects of ozone have been reported on aphids, which were in all cases mediated by the plants. In this study we evaluated the effect of direct exposure to ozone on Metopolophium dirhodum (Hemiptera: Aphididae). Insects fed on artificial diets were exposed to ozone in open top chambers (OTCs) at three doses: 166,6  1,3 ppb, 45,3  1,9 ppb and 0,1  0,1ppb during 6 hours. After exposure, insects were recounted and the proportions of dead and escaped insects were calculated. Lipid peroxidation (TBARS) and total reactive antioxidant potential (TRAP) were assessed as oxidative stress markers on the surviving insects. Our results show that ozone directly affected both aphid behavior and population size: the proportion of insects escaping from diet cages was highest in the control treatment (F2, 25= 4,15, p=0,0278; DMS Tukey 25 gl, α:0,05 = 0,069). Insect mortality was higher in both ozone doses than in the control treatment (F2, 25= 10,01, p=0,0006; DMS Tukey 25 gl, α:0,05 = 0,111, DMS Tukey 25 gl, α:0,1 = 0,097). Additionally, TBARS were higher at 166 ppb than in the 45 ppb treatment, although not different from the control (F2,8= 8,55, p= 0,0103, DMS Tukey 8 gl, α:0,05 = 0,402). Besides, no significant differences were detected on aphid TRAP among ozone treatments (F2,8=0,68, p= 0,5310). Ozone exposure negatively affected aphids. These results on the direct effect of ozone on insects add relevant insights for the understanding on how this pollutant affects plant-aphid interaction.