ALS herbicide resistance mutations in Raphanus raphanistrum: Evaluation of pleiotropic effects on vegetative growth and ALS activity

LI, M; YU Q; HAN, H; VILA AIUB MM; POWLES, SB

PEST MANAGEMENT SCIENCE

JOHN WILEY & SONS LTD

Lugar: LOndres; Año: 2013 p. 689 - 695

1526-498X

BACKGROUND:Gene mutations that endow herbicide resistance may cause pleiotropic effects on plant ecology and physiology. This paper reports on the effect of a number of known and novel target-site resistance mutations of the ALS gene (Ala-122-Tyr,Pro-197-Ser, Asp-376-Glu or Trp-574-Leu) on vegetative growth traits of the weed Raphanus raphanistrum . RESULTS:The results from a series of experiments have indicated that none of these ALS resistance mutations imposes negative pleiotropic effects on relative growth rate (RGR), photosynthesis and resource-competitive ability in R. raphanistrum plants. The absence of pleiotropic effects on plant growth occurs in spite of increased (Ala-122-Tyr, Pro-197-Ser, Asp-376-Glu) and decreased (Trp-574-Leu) extractable ALS activity. CONCLUSION: The absence of detrimental pleiotropic effects on plant growth associated with the ALS target-site resistance mutations reported here is a contributing factor in resistance alleles being at relatively high frequencies in ALS-herbicide-unselected R.raphanistrum populations