CCR5 AND CCR2 POLYMORPHISMS AND THEIR ASSOCIATION WITH CHRONIC CHAGAS CARDIOMYOPATHY (CCC) IN ARGENTINIAN POPULATION

CLARA I GONZALEZ; GISELA MORALES SANFURGO; DANIEL HERNÁNDEZ; LONGHI, SILVIA A.; ALEJANDRA GARCILAZO; NATALIA JUIZ; SCHIJMAN ALEJANDRO G; RAUL CHADI; ELKYN ESTUPIÑÁN

Buenos Aires

Congreso; REUNIÓN CONJUNTA DE SOCIEDADES DE BIOCIENCIAS-XXIX REUNIÓN ANUAL DE LA SOCIEDAD ARGENTINA DE PROTOZOOLOGÍA; 2017

Sociedad Argentina de Protozoologia

Several studies have proposed different genetic markers as chemokinesand cytokines genes for susceptibility to develop CCC.Many genes may be involved, each one making a small contribution.Thus, an appropriate approach for this problematic is to studynumerous SNPs in individuals sharing genetic background.Our aim was to analyze CCR2 and CCR5 SNPs located in thepromoter region by TaqMan allelic discrimination assay, and theirassociation with CCC in Argentinean populations.A case-control study was carried in 480 T. cruzi seropositive adultsfrom Gran Chaco endemic region and patients attending BuenosAires hospitals. They were classified in 2 groups according to theConsensus on Chagas-Mazza Disease: non-demonstrated (non-DC) or demonstrated (DC) pathology groups.Due to our studied population did not fit Hardy-Weimberg equilibrium,we subclassified them according to geographical/ethnicorigin. Thereby, SNPs frequencies between creole population fromendemic regions and Buenos Aires patients were similar but differfrom wichi population from Gran Chaco. The association analysisshowed that the T allele in rs1800024 was more represented in non-DC than in DC group (p=0.041) in non-wichi population, becoming aprotective factor. Among wichi individuals the G allele in rs1800023was more frequent in DC group (p=0.016) and may be a risk factorto CCC. Moreover, we found that only in wichi population the HHEhaplotype displayed a higher prevalence in non-DC group.These results are consistent with a previous study showing thatalthough wichi and creoles live in the same geographical area, theyare genetically different and for this reason, the results differed accordingto the population studied. It is tempting to speculate an associationbetween the above described genetic differences and theclinical manifestations of CCC. Indeed, right bundle-branch block,the most frequent abnormality in CCC, had a clear tendency forlower prevalence in the wichi population.