INGEBI   02650
INSTITUTO DE INVESTIGACIONES EN INGENIERIA GENETICA Y BIOLOGIA MOLECULAR "DR. HECTOR N TORRES"
Unidad Ejecutora - UE
congresos y reuniones científicas
Título:
Functional changes in synaptic transmission at the mouse medial olivocochlear (MOC)-inner hair cell synapse during neonatal development
Autor/es:
GRACIELA KEARNEY; LUCAS VATTINO; CAROLINA WEDEMEYER; JAVIER ZORRILLA DE SAN MARTÍN; ANA BELÉN ELGOYHEN; ELEONORA KATZ
Lugar:
Mar del Plata
Reunión:
Congreso; XXX Congreso Anual de la Sociedad Argentina de Investigación en Neurociencias (SAN); 2015
Institución organizadora:
SAN
Resumen:
Inner hair cells (IHC) are innervated by MOC fibers since birth to the onset of hearing(postnatal day 12, P12). At P9-11 ACh release is supported by P/Q- and N-type VGCC andnegatively regulated by L-type VGCC coupled to BK channels. The transient nature of theMOC-IHC synapse suggests there might be changes in the properties of synaptictransmission throughout this period.Short term synaptic plasticity (STP) is the change in synaptic strength(facilitation/depression) as a consequence of preceding activity. We have shown thatstimulation trains at 10, 40 and 100 Hz led to depression at P9-11 whereas at P6-7 the 10Hz train led to facilitation and the 40 and 100 Hz trains to facilitation followed bydepression. We now found that at P4 synapses trains at 10, 40 and 100 Hz caused a1.5±0.7; 2.2±0.6 and 2.0±0.6-fold increase, respectively, in the ratio between theamplitude of the tenth and the first evoked synaptic current, indicating that at this stagethere is facilitation even at the highest frequencies tested. Consistently, both the quantalcontent (0.3±0.1 P4; 0.8±0.1 P6-7; 1.6±0.2 P9-11 p