Subcellular localization and tissue expression analysis of StCDPK7, a CDPK isoform related to biotic stress

FANTINO, ELISA; SANTIN FRANCO; SCIORRA, M; ULLOA RITA M

Foz de Iguazu

Congreso; XI International Plamt Molecular Biology Meeting; 2015

IPMB, comité binacional Argentina-Brasil

Calcium dependent protein kinases (CDPKs) are key components of calcium regulated signaling cascades in plants. CDPKs combine Ca2+ sensing (EF hand motifs) and a responding function (protein kinase activity) within a single protein and have therefore been classified as sensor responders. These kinases translate information encoded in the Ca2+ signatures into phosphorylation events of specific target proteins which are involved in metabolism, osmosis, hormone response, immune and stress signaling networks. In potato, the CDPK family is composed of twenty-eight members, only five of them are well characterized (Raíces et al., 2001; Gargantini et al., 2009, Giammaria et al., 2011; Grandellis et al., 2012, Kobayashi et al., 2007). All of them differ in their N-terminal variable (NTV) domain, which regulates its subcellular localization. Isoform StCDPK7, from group I, was cloned and the recombinant protein characterized previously. Our aim is to continue studying this enzyme and elucidate its potential role in plant defense responses.