Genetic analysis of the regulation of pollen mRNA storage in cytoplasmic bodies

MUSCHIETTI, JORGE; SCARPIN , REGINA; SIGAUT, LORENA; PIETRASANTA, LIA

Foz

Congreso; IPMB2015; 2015

Genetic Analysis of the Regulation of Pollen mRNA Storage in Cytoplasmic Bodies Jorge Muschietti1, Lorena Sigaut2, Lia Pietrasanta2 and Maria Regina Scarpin1. 1INGEBI-CONICET, DBBE-FCEN, University of Buenos Aires. 2Centro de Microscopías Avanzadas, DF-FCEN, University of Buenos Aires.It has been shown that most of the pollen proteins are synthetized during pollen development, while the rest is produced after germination and during pollen tube growth. However, there are reports that some pollen mRNAs are synthesized during pollen development, accumulated in dehydrated mature pollen grains but translated only after pollen germinates. Our hypothesis is that accumulation of these pre-synthesized pollen mRNAs is mediated by cytoplasmic bodies. In order to probe this, we analyzed the cellular localization of some defined pollen pre-synthesized mRNAs during pollen development using the MS2-CP system. We studied stress granules (SGs) and processing bodies (PBs) in Arabidopsis mature pollen as possible cellular structures where these pre-synthesized pollen mRNAs can be stored before being translated. Our results would contribute to understand the mechanisms of transcriptional and translational regulation in Arabidopsis thaliana pollen grains.